Orotic acid

Orotic acid (6-Carboxyuracil), a precursor in biosynthesis of pyrimidine nucleotides and RNA, is released from the mitochondrial dihydroorotate dehydrogenase (DHODH) for conversion to UMP by the cytoplasmic UMP synthase enzyme. Orotic acid is a marker for measurement in routine newborn screening for urea cycle disorders. Orotic acid can induce hepatic steatosis and hepatomegaly in rat.

Product information

CAS Number: 65-86-1

Molecular Weight: 156.10

Formula: C5H4N2O4

Synonym:

6-Carboxyuracil

Vitamin B13

Chemical Name: 2, 6-dioxo-1, 2, 3, 6-tetrahydropyrimidine-4-carboxylic acid

Smiles: OC(=O)C1=CC(=O)NC(=O)N1

InChiKey: PXQPEWDEAKTCGB-UHFFFAOYSA-N

InChi: InChI=1S/C5H4N2O4/c8-3-1-2(4(9)10)6-5(11)7-3/h1H,(H,9,10)(H2,6,7,8,11)

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO: 2 mg/mL(12.81 mM). Water: Insoluble.

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Orotic acid is found in milk and dairy products, and it is converted to uridine for use in the pyrimidine salvage pathway predominantly in liver, kidney and erythrocytes.

In Vivo:

Orotic acid is a marker for measurement of urea cycle disorders (UCDs), including ornithine transcarbamylase deficiency (OTCD). Orotic acid (1.0% addition to the diet; p.o. for 3-10 d) induces a development of fatty liver by day 7, and decreases purine/pyrimidine ratio of hepatic acid-soluble nucleotides by day 3.

References:

1. Löffler M, et, al. Orotate (orotic acid): An essential and versatile molecule. Nucleosides Nucleotides Nucleic Acids.
2. Staretz-Chacham O, et, al. The role of orotic acid measurement in routine newborn screening for urea cycle disorders. J Inherit Metab Dis. 2020 Nov 15.
3. Durschlag RP, et, al. Orotic acid-induced metabolic changes in the rat. J Nutr. 1980 Apr;110(4):816-21.

Products are for research use only. Not for human use.