5(6)-FITC

5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.

Product information

CAS Number: 27072-45-3

Molecular Weight: 778.76

Formula: C42H22N2O10S2

Synonym:

Fluorescein 5(6)-isothiocyanate

FITC

Fluorescein isothiocyanate 5- and 6- isomers

Chemical Name: 3', 6'-dihydroxy-5(6)-isothiocyanato-3H-spiro[2-benzofuran-1, 9'-xanthen]-3-one

Smiles: OC1=CC2OC3=CC(O)=CC=C3C3(OC(=O)C4=CC=C(C=C34)N=C=S)C=2C=C1.OC1=CC2OC3=CC(O)=CC=C3C3(OC(=O)C4=CC(=CC=C34)N=C=S)C=2C=C1

InChiKey: AEWCEHLYVLRLBF-UHFFFAOYSA-N

InChi: InChI=1S/2C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21;23-12-2-5-15-18(8-12)26-19-9-13(24)3-6-16(19)21(15)17-7-11(22-10-28)1-4-14(17)20(25)27-21/h2*1-9,23-24H

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : ≥ 156 mg/mL (400.64 mM)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

References:

1. Goding JW. Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Methods. 1976;13(3-4):215-26.
2. Der-Balian GP, et al. Fluorescein labeling of Fab' while preserving single thiol. Anal Biochem. 1988 Aug 15;173(1):59-63.
3. Smith LM, et al. Fluorescence detection in automated DNA sequence analysis. Nature. 1986 Jun 12-18;321(6071):674-9.

Products are for research use only. Not for human use.