ATN-224

Bis-choline tetrathiomolybdate is a cytosolic superoxide dismutase (SOD1) inhibitor potentially for the treatment of Wilson's disease.

Product information

CAS Number: 649749-10-0

Molecular Weight: 434.56

Formula: C10H30MoN2O2S4

Synonym:

ATN224

ATN 224

Bis(choline)tetrathiomolybdate

Chemical Name: bis((2-hydroxyethyl)trimethylazanium) disulfanylidenemolybdenum disulfanide

Smiles: [SH-].[SH-].C[N+](C)(C)CCO.C[N+](C)(C)CCO.S=[Mo]=S

InChiKey: FSWNJZRQQVVVJT-UHFFFAOYSA-L

InChi: InChI=1S/2C5H14NO.Mo.2H2S.2S/c2*1-6(2,3)4-5-7;;;;;/h2*7H,4-5H2,1-3H3;;2*1H2;;/q2*+1;;;;;/p-2

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: Soluble in water

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

ATN-224 induced peroxynitrite-dependent cell death, independent of Bcl-2 status, at nanomolar concentrations in cell culture models of T cell lymphoid malignancies. To determine the ability of ATN-224 to induce cell death in primary patient samples, ATN-224 was tested in pre-treatment, precursor B cell acute lymphoblastic leukemia (B-ALL), which commonly overexpresses Bcl-2. Five B-ALL samples were treated with 12.5 nM and 25 nM concentrations of ATN-224 for 24 h. A decrease in the number of viable cells in all five B-ALL samples was observed (Figure 7). There appears to be a concentration dependent decrease occurring in most of the samples, with responses ranging from 11–51% at 12.5 nM and 14–46% at 25 nM. These results imply that ATN-224 has therapeutic potential in the treatment of B cell lymphoid malignancies, and may be particularly useful in those malignancies which overexpress Bcl-2.

References:

1. Lee K, Briehl MM, Mazar AP, Batinic-Haberle I, Reboucas JS, Glinsmann-Gibson B, Rimsza LM, Tome ME. The copper chelator ATN-224 induces peroxynitrite-dependent cell death in hematological malignancies. Free Radic Biol Med. 2013 Jul;60:157-67. doi: 10.1016/j.freeradbiomed.2013.02.003. Epub 2013 Feb 14. PMID: 23416365; PMCID: PMC3654089. 2. Lee K, Hart MR, Briehl MM, Mazar AP, Tome ME. The copper chelator ATN-224 induces caspase-independent cell death in diffuse large B cell lymphoma. Int J Oncol. 2014 Jul;45(1):439-47.

Products are for research use only. Not for human use.