Zinc Protoporphyrin

Zinc Protoporphyrin (Zn(II)-protoporphyrin IX) is an orally active and competitive heme oxygenase-1 (HO-1) inhibitor and markedly attenuates the protective effects of Phloroglucinol (PG) against H2O2. Zinc Protoporphyrin is used as a screening marker of iron deficiency in individual pregnant women and children, but also to assess population iron status in combination with haemoglobin concentration. Zinc Protoporphyrin has anti-cancer activity.

Product information

CAS Number: 15442-64-5

Molecular Weight: 626.05

Formula: C34H32N4O4Zn

Synonym:

Zn(II)-protoporphyrin IX

ZnPP

Zinc Protoporphyrin-9

Chemical Name: zinc;3-[18-(2-carboxyethyl)-8, 13-bis(ethenyl)-3, 7, 12, 17-tetramethylporphyrin-21, 23-diid-2-yl]propanoic acid

Smiles: [Zn+2].CC1=C(C=C)C2C=C3[N-]C(=CC4=NC(=CC5[N-]C(C=C1N=2)=C(C)C=5CCC(O)=O)C(CCC(O)=O)=C4C)C(C=C)=C3C

InChiKey: FUTVBRXUIKZACV-RGGAHWMASA-L

InChi: InChI=1S/C34H34N4O4.Zn/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);/q;+2/p-2/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 50 mg/mL (79.87 mM; Need ultrasonic)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Zinc Protoporphyrin (Zn(II)-protoporphyrin IX; 5 μM; 72 hours) causes the fraction of late apoptotic and necrotic cells increasing from 10.9% in controls to 30.4% after 72 h. Zinc Protoporphyrin (1.25-40 μM; 48 or 72 hours) exerts cystostatic/cytotoxic effects against tumor cells. Zinc Protoporphyrin (2.5, 5 μM; 48 or 72 hours) results in dose- and time-dependent reduction of cells in G1 phase of the cell cycle. Zinc Protoporphyrin (1.25-40 μM; 48 hours) leads to accumulation of cleaved (active) caspase-3.

In Vivo:

Zinc Protoporphyrin (12.5, 25, 50 mg/kg for i.p.; 12.5, 50 mg/kg for p.o.; from day 7 to 19) exerts dose-dependent antitumor effects manifested by the retardation of tumor growth.

References:

1. Park C, et al. Protective Effect of Phloroglucinol on Oxidative Stress-Induced DNA Damage and Apoptosisthrough Activation of the Nrf2/HO-1 Signaling Pathway in HaCaT Human Keratinocytes. Mar Drugs. 2019 Apr 13;17(4).
2. Mwangi MN, et al. Diagnostic utility of zinc protoporphyrin to detect iron deficiency in Kenyan pregnant women. BMC Med. 2014 Nov 26;12:229.
3. Nowis D, et al. Zinc protoporphyrin IX, a heme oxygenase-1 inhibitor, demonstrates potent antitumor effects but is unable to potentiate antitumor effects of chemotherapeutics in mice. BMC Cancer. 2008 Jul 11;8:197.

Products are for research use only. Not for human use.