(S)-10-Hydroxycamptothecin

(S)-10-Hydroxycamptothecin (10-HCPT;10-Hydroxycamptothecin) is a DNA topoisomerase I inhibitor of isolated from the Chinese plant Camptotheca accuminata. (S)-10-Hydroxycamptothecin exhibits a remarkable apoptosis-inducing effect. (S)-10-Hydroxycamptothecin has the potential for hepatoma, gastric carcinoma, colon cancer and leukaemia treatment.

Product information

CAS Number: 19685-09-7

Molecular Weight: 364.35

Formula: C20H16N2O5

Synonym:

10-HCPT

10-Hydroxycamptothecin

Chemical Name: (19S)-19-ethyl-7, 19-dihydroxy-17-oxa-3, 13-diazapentacyclo[11.8.0.0, .0, .0, ]henicosa-1(21), 2, 4, 6, 8, 10, 15(20)-heptaene-14, 18-dione

Smiles: CC[C@@]1(O)C(=O)OCC2C(=O)N3CC4=CC5=CC(O)=CC=C5N=C4C3=CC1=2

InChiKey: HAWSQZCWOQZXHI-FQEVSTJZSA-N

InChi: InChI=1S/C20H16N2O5/c1-2-20(26)14-7-16-17-11(5-10-6-12(23)3-4-15(10)21-17)8-22(16)18(24)13(14)9-27-19(20)25/h3-7,23,26H,2,8-9H2,1H3/t20-/m0/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 50 mg/mL (137.23 mM; Need ultrasonic)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

(S)-10-Hydroxycamptothecin (5-20 µg/L; 6 days; Hep G2 cells) treatment results in the cell cycle arrest at G2/M phase. (S)-10-Hydroxycamptothecin induces differentiation, down-regulates nuclear antigen (PCNA) and up-regulates wild-type protein p53 in Hep G2 cells. (S)-10-Hydroxycamptothecin inhibits L1210 leukemia cells with an IC50 of 1.15 µM.

In Vivo:

(S)-10-Hydroxycamptothecin (10-Hydroxycamptothecin) against L1210 leukemia in mice is tested. At the optimal dose (15 mg/kg), (S)-10-Hydroxycamptothecin has a 71% increase in life span (ILS).

References:

1. Min Liu, et al. Intracellular delivery of 10-hydroxycamptothecin with targeted nanostructured lipid carriers against multidrug resistance . Journal of Drug Targeting.
2. Min Liu, et al. Intracellular delivery of 10-hydroxycamptothecin with targeted nanostructured lipid carriers against multidrug resistance. J Drug Target, Early Online: 1–8
3. Yu P, et al. Synthesis and preliminary anticancer evaluation of 10-hydroxycamptothecin analogs. Biol Pharm Bull. 2012;35(8):1295-9.
4. Zhang XW, et al. Differentiation-inducing action of 10-hydroxycamptothecin on human hepatoma Hep G2 cells. Acta Pharmacol Sin. 2000 Apr;21(4):364-8.

Products are for research use only. Not for human use.