Bz 423

Bz 423 is a pro-apoptotic 1, 4-benzodiazepine with therapeutic properties in murine models of lupus demonstrating selectivity for autoreactive lymphocytes, and activates Bax and Bak.

Product information

CAS Number: 216691-95-1

Molecular Weight: 440.92

Formula: C27H21ClN2O2

Synonym:

BZ48

Chemical Name: 7-chloro-5-(4-hydroxyphenyl)-1-methyl-3-[(naphthalen-2-yl)methyl]-2, 3-dihydro-1H-1, 4-benzodiazepin-2-one

Smiles: CN1C(=O)C(CC2=CC3=CC=CC=C3C=C2)N=C(C2=CC(Cl)=CC=C12)C1C=CC(O)=CC=1

InChiKey: HIJCSNHREFFOML-UHFFFAOYSA-N

InChi: InChI=1S/C27H21ClN2O2/c1-30-25-13-10-21(28)16-23(25)26(19-8-11-22(31)12-9-19)29-24(27(30)32)15-17-6-7-18-4-2-3-5-20(18)14-17/h2-14,16,24,31H,15H2,1H3

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : ≥ 125 mg/mL (283.50 mM)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Bz-423 induces a larger superoxide response in Ramos cells, and causes rapid death of Ramos B cells. Bz-423 activates Bax and Bak, but does not activate ASK1/JNK in Ramos cells. In Ramos cells Bz-423-induced superoxide leads to Bax and Bak activation via changes in Mcl-1 expression and functional activation of BH3-only proteins. Bz-423 rapidly increases superoxide levels in MEFs within 1 h and the magnitude of the increase is concentration-dependent. Bz-423-induced apoptosis dependents upon superoxide.

References:

1. Blatt NB, et al. Bz-423 superoxide signals B cell apoptosis via Mcl-1, Bak, and Bax. Biochem Pharmacol. 2009 Oct 15;78(8):966-73.
2. Blatt NB, et al. Bz-423 superoxide signals apoptosis via selective activation of JNK, Bak, and Bax. Free Radic Biol Med. 2008 Nov 1;45(9):1232-42.

Products are for research use only. Not for human use.