Y-27632, Rock Inhibitor.

Y27632 is a selective ROCK inhibitor, which inhibits ET-1-induced increases in natriuretic peptide production, cell size, protein synthesis, and myofibrillar organization. Y27632 prevents dimethylnitrosamine-induced hepatic fibrosis in rats, increases apoptosis and disrupts the actin cortical mat in embryonic avian corneal epithelium, affects initial heart myofibrillogenesis in cultured chick blastoderm, promotes the proliferation and cell cycle progression of cultured astrocyte from spinal cord.

Product information

CAS Number: 146986-50-7

Molecular Weight: 247.34

Formula: C14H21N3O

Chemical Name: (1R,4r)-4-((R)-1-aminoethyl)-N-(pyridin-4-yl)cyclohexanecarboxamide

Smiles: C[C@@H](N)[C@@H]1CC[C@H](CC1)C(=O)NC1C=CN=CC=1

InChiKey: IYOZTVGMEWJPKR-IJLUTSLNSA-N

InChi: InChI=1S/C14H21N3O/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13/h6-12H,2-5,15H2,1H3,(H,16,17,18)/t10-,11-,12-/m1/s1

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: (25°C) DMSO: 64 mg/mLwarmed(199.83 mM). Water: 14 mg/mL(43.71 mM).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

Y-27632 is typically used at 10 μM concentration in cell culture.

In Vivo:

In several hypertension rat models, Y-27632 was orally dosed at 30 mg/kg to significantly decrease blood pressure.

References:

1. Uehata M, et al. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension.(1997) Nature. 389(6654):990-4.
2. Ishizaki T. et al. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. (2000) Mol Pharmacol 57, 976-983.
3. Koyanagi et al. Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. (2008) J. Neurosci Res 86: 270-280.
4. Watanabe et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. (2007) Nat Biotech 25: 681-686.

Products are for research use only. Not for human use.