详情描述
5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate; CFDA) is a cell-permeant, amine-reactive green fluorescent probe used to stain cells for analysis in applications. (λex: 492 nm; λem: 517 nm).
Product information
CAS Number: 124387-19-5
Molecular Weight: 460.39
Formula: C50H32O18
Synonym:
5-(6)-Carboxyfluorescein diacetate
CFDA
Chemical Name: 3',6'-bis(acetyloxy)-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-6-carboxylic acid; 3',6'-bis(acetyloxy)-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-7-carboxylic acid
Smiles: CC(=O)OC1=CC2OC3=CC(=CC=C3C3(OC(=O)C4=CC=CC(=C34)C(O)=O)C=2C=C1)OC(C)=O.CC(=O)OC1=CC2OC3=CC(=CC=C3C3(OC(=O)C4=CC=C(C=C34)C(O)=O)C=2C=C1)OC(C)=O
InChiKey: SWRGCNMDGRMQGB-UHFFFAOYSA-N
InChi: InChI=1S/2C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25;1-12(26)31-14-6-8-18-20(10-14)33-21-11-15(32-13(2)27)7-9-19(21)25(18)22-16(23(28)29)4-3-5-17(22)24(30)34-25/h2*3-11H,1-2H3,(H,28,29)
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 125 mg/mL (271.51 mM; Need ultrasonic)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
References:
- Yang T, et al. A novel nonradioactive CFDA assay to monitor the cellular immune response in myeloid leukemia. Immunobiology. 2013 Apr;218(4):548-53.
- Card SD, et al. Assessment of fluorescein-based fluorescent dyes for tracing Neotyphodium endophytes in planta. Mycologia. 2013 Jan-Feb;105(1):221-9.
- Fang X, et al. Bone marrow-derived endothelial progenitor cells are involved in aneurysm repair in rabbits. J Clin Neurosci. 2012 Sep;19(9):1283-6.
Products are for research use only. Not for human use.