Grape seed extract

Grape seed extract is a natural product, with anti-inflammatory and anti-proliferative effects. Grape seed extract shows inhibitory activity on the fat-metabolizing enzymes pancreatic lipase and lipoprotein lipase. Grape seed extract induces apoptotic in human colorectal cancer cells.

Product information

CAS Number: 84929-27-1

Molecular Weight: 590.57

Formula: C32H30O11

Chemical Name: (2R, 3S, 4S)-2-(3, 4-dihydroxyphenyl)-4-[(2R, 3R)-2-(3, 4-dihydroxyphenyl)-3, 7-dihydroxy-3, 5-dimethyl-2, 4-dihydrochromen-8-yl]-3, 4-dihydro-2H-chromene-3, 5, 7-triol

Smiles: CC1=CC(O)=C(C2C(O)C(OC3=CC(O)=CC(O)=C32)C2C=CC(O)=C(O)C=2)C2OC(C3=CC(O)=C(O)C=C3)C(C)(O)CC1=2

InChiKey: VWKAFYWVDIOMSL-UHFFFAOYSA-N

InChi: InChI=1S/C32H30O11/c1-13-7-22(38)26(30-17(13)12-32(2,41)31(43-30)15-4-6-19(35)21(37)9-15)27-25-23(39)10-16(33)11-24(25)42-29(28(27)40)14-3-5-18(34)20(36)8-14/h3-11,27-29,31,33-41H,12H2,1-2H3

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 6 mg/mL (Need ultrasonic)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Grape seed extract (25-100 μg/mL; 12-48 hours) inhibits growth and induces death in human colorectal cancer cells. Grape seed extract (25-100 μg/mL; 12-24 hours) modulates the protein levels of cell cycle regulatory molecules in HT29 and LoVo cells. Grape seed extract (25-100 μg/mL; 24 hours) causes apoptotic cell death of human colorectal cancer cells.

In Vivo:

Grape seed extract (200 mg/kg; i.g.; 5 days/wk; for 8 weeks) inhibits HT29 colon carcinoma tumor xenograft growth in athymic nude mice. Grape seed extract inhibits cell proliferation but increases apoptotic cell death in tumors. Grape seed extract enhances Cip1/p21protein levels and poly(ADP-ribose) poly-merase cleavage in tumors.

References:

1. The effects of grape seed extract (Vitis vinifera) supplement on inflammatory markers, neuropeptide Y, anthropometric measures, and appetite in obese or overweight individuals: A randomized clinical trial.
2. Inhibitory effects of grape seed extract on lipases. Nutrition. 2003 Oct;19(10):876-9.
3. Manjinder Kaur, et al. Grape seed extract inhibits in vitro and in vivo growth of human colorectal carcinoma cells. Clin Cancer Res. 2006 Oct 15;12(20 Pt 1):6194-202.

Products are for research use only. Not for human use.