HM Janelia Fluor® 526, SE

HM Janelia Fluor® 526, SE is a spontaneously blinking green-emitting dye and a hydroxymethyl derivative of JF526. It is suitable for super-resolution imaging including dSTORM, STED and single-molecule localization spectroscopy (SMLSM). The spontaneous blinking properties of HM Janelia Fluor® 526, avoids the requirement for strongly reducing dSTORM buffers. It is also suitable for conventional confocal microscopy and live cell imaging. Fluorogenic spontaneously blinking green-emitting dye; hydroxymethyl derivative of JF526. Suitable for super-resolution imaging including dSTORM, STED and single-molecule localization spectroscopy (SMLSM). The spontaneous blinking properties of HM Janelia Fluor® 526, avoids the requirement for strongly reducing dSTORM buffers. Also suitable for conventional confocal microscopy and live cell imaging. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.

Product information

CAS Number: 2376841-30-2

Molecular Weight: 645.51

Formula: C31H21F6N3O6

Synonym:

HM-JF526 NHS

HMJF526 NHS

HM JF526 NHS

Chemical Name: 2,5-dioxopyrrolidin-1-yl 3',6'-bis(3,3-difluoroazetidin-1-yl)-2',7'-difluoro-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylate

Smiles: O=C(ON1C(=O)CCC1=O)C1=CC=C2COC3(C2=C1)C1=CC(F)=C(C=C1OC1=CC(=C(F)C=C31)N1CC(F)(F)C1)N1CC(F)(F)C1

InChiKey: HJUSNAUMCROPEK-UHFFFAOYSA-N

InChi: InChI=1S/C31H21F6N3O6/c32-20-6-18-24(8-22(20)38-11-29(34,35)12-38)45-25-9-23(39-13-30(36,37)14-39)21(33)7-19(25)31(18)17-5-15(1-2-16(17)10-44-31)28(43)46-40-26(41)3-4-27(40)42/h1-2,5-9H,3-4,10-14H2

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: Soluble in DMSO

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

Products are for research use only. Not for human use.