6-TAMRA-SE

6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing.

Product information

CAS Number: 150810-69-8

Molecular Weight: 527.52

Formula: C29H25N3O7

Synonym:

6-TAMRA-NHS ester

6-Carboxytetramethylrhodamine N-succinimidyl ester

Chemical Name: 9-(2-carboxylato-5-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}phenyl)-3,6-bis(dimethylamino)-10λ⁴-xanthen-10-ylium

Smiles: CN(C)C1C=C2[O+]=C3C=C(C=CC3=C(C3=CC(=CC=C3C([O-])=O)C(=O)ON3C(=O)CCC3=O)C2=CC=1)N(C)C

InChiKey: PAOQTZWNYMMSEE-UHFFFAOYSA-N

InChi: InChI=1S/C29H25N3O7/c1-30(2)17-6-9-20-23(14-17)38-24-15-18(31(3)4)7-10-21(24)27(20)22-13-16(5-8-19(22)28(35)36)29(37)39-32-25(33)11-12-26(32)34/h5-10,13-15H,11-12H2,1-4H3

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 100 mg/mL (189.57 mM; Need ultrasonic)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

6-TAMRA-SE (6-TAMRA-NHS ester) is an amine-reactive form of tetramethylrhodamine. 6-TAMRA-SE (soluble in DMF and DMSO, absorption=546, emission=576 measured in MeOH ϵ=9.5×104ϵ=9.5×104).

References:

1. Thomas TP, et al. Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe. J Biomed Opt. 2008;13(1):014024.
2. Tessmar J, et al. Toward the development of biomimetic polymers by protein immobilization: PEGylation of insulin as a model reaction. Tissue Eng. 2004;10(3-4):441-453.
3. Wang Y, et al. RNA molecules that specifically and stoichiometrically bind aminoglycoside antibiotics with high affinities. Biochemistry. 1996 Sep 24;35(38):12338-46.

Products are for research use only. Not for human use.